DNA

Part:BBa_K4231011:Design

Designed by: yang qingyan   Group: iGEM22_USTC   (2022-09-30)


knock box of URA3 gene in yarrowia lipolytica


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 136
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 136
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no extra base pair in 5' end and 3' end


Source

conjunct about 400 bp sequence from each end of URA3 select marker

References